EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    4.1.1.45 | -999 |
- |
- |
4568 |
| 4.1.1.45 | -999 |
- |
3.07 micromol/h/g kidney, enzyme assay performed at 25°C, pH 8.0 and 50 mmol/l Tris-acetate buffer |
666971 |
| 4.1.1.45 | -999 |
- |
9.3 micromol/h/g liver, enzyme assay performed at 25°C, pH 8.0 and in 50 mmol/l Tris-acetate buffer |
666971 |
| 4.1.1.45 | -999 |
- |
ACMSD assay at pH 7.0 and in 50 mM potassium phosphate buffer. ACMSD activity is suppressed 20fold and 4fold after feeding of rats with linoleic acid concentrations of 20% and 10% respectively compared to 40 micromol/h/g protein without feeding of linoleic acid. Palmitic acid, stearic acid and oleic acid do not suppress ACMSD activity. N-3 fatty acids suppress ACMSD activity even at only 2% in diets. |
664173 |
| 4.1.1.45 | 0.006 |
- |
crude cell extract, in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C |
679800 |
| 4.1.1.45 | 0.36 |
- |
enzyme assay performed at 25°C, pH 8.0 and 50 mmol/l Tris-acetate buffer |
666971 |
| 4.1.1.45 | 0.8 |
- |
25 mM Hepes buffer at pH 7.0 and 25°C, under not saturating conditions. ACSMD loaded with 1 molar equivalent Mn2+ |
665303 |
| 4.1.1.45 | 0.8 |
- |
recombinant enzyme, pH 7.0, temperature not specified in the publication |
749250 |
| 4.1.1.45 | 1.39 |
- |
after 231fold purification, in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C |
679800 |
| 4.1.1.45 | 1.7 |
- |
25 mM Hepes buffer at pH 7.0 and 25°C, under not saturating conditions. ACSMD loaded with 1 molar equivalent Cd2+ |
665303 |
