EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
3.6.4.10 | -999 |
- |
high-throughput screening method in 96-well plates for ATPase activity of DnaK. Screening of potential inhibitors |
684406 |
3.6.4.10 | -999 |
- |
mutation T213N or T612N produce a ca. 50% decrease in the ATPase activity, in mutant T213N/T612N the ATPase activity was approximately sevenfold lower than that of wild type ClpB, the ATPase of all ClpB variants is activated by soluble pseudosubstrates (casein and poly-lysine) |
701108 |
3.6.4.10 | 0.0000026 |
- |
- |
210500 |
3.6.4.10 | 0.00086 |
- |
purified recombinant enzyme, pH 7.5, 37°C |
735005 |
3.6.4.10 | 0.00167 |
- |
purified recombinant enzyme, pH 7.5, 25°C, in absence of activators |
734233 |
3.6.4.10 | 0.00283 |
- |
purified recombinant enzyme, pH 7.5, 25°C, in presence of 0.006 mM HSC20 |
734233 |
3.6.4.10 | 0.0075 |
- |
purified recombinant enzyme, pH 7.5, 25°C, in presence of 0.015 mM ISCU |
734233 |
3.6.4.10 | 0.025 |
- |
purified recombinant enzyme, pH 7.5, 25°C, in presence of 0.004 mM HSC20 and 0.015 mM ISCU |
734233 |
3.6.4.10 | 0.412 |
- |
purified recombinant enzyme, pH 7.4, temperature not specified in the publication |
734478 |