EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   3.5.5.1 | -999 |
- |
after 20.3fold purification, the specific activity is 144 units/mg, while the crude enzyme exhibits a specific activity of 7.1 units/mg at pH 8.0 and 45°C |
713440 |
| 3.5.5.1 | 0.0006 |
- |
pH 7.4, 70°C, substrate: benzonitrile |
724954 |
| 3.5.5.1 | 0.0037 |
- |
pH 7.0, 90°C, substrate: 2-cyanopyridine |
756683 |
| 3.5.5.1 | 0.075 |
- |
cell extract, pH 9.0, 30°C |
718611 |
| 3.5.5.1 | 0.1 |
- |
enzyme in cells, substrate benzonitrile in Na/K-phosphate buffer, pH 7.0, 30°C |
718648 |
| 3.5.5.1 | 0.1 |
- |
the specific activity is less than 0.1 micromol/min/mg, wild type enzyme, using (R)-2-chloromandelonitrile as substrate, in 50 mM potassium phosphate buffer, at pH 4.5 and 30°C |
711870 |
| 3.5.5.1 | 0.12 |
- |
benzonitrilase B |
209758 |
| 3.5.5.1 | 0.14 |
- |
mutant enzyme A198V/I290F/H135Y/Y213H/T350S/Y177C/A285T, using (R)-2-chloromandelonitrile as substrate, in 50 mM potassium phosphate buffer, at pH 7.5 and 30°C |
711870 |
| 3.5.5.1 | 0.24 |
- |
recombinant enzyme expressed without GroEL/ES, pH 8.0, 30°C |
718670 |
| 3.5.5.1 | 0.28 |
- |
recombinant enzyme expressed without GroEL/ES, pH 8.0, 30°C |
718670 |
