EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   3.4.24.75 | -999 |
- |
- |
31508, 31511, 650275, 653149, 653739 |
| 3.4.24.75 | -999 |
- |
11900 units per mg, after 12 fold purification, 1 unit is defined as the amount of enzyme required to decrease the OD(A600) of a suspension of Staphylococcus aureus 237 cells by 0.001 at 37°C in 5 min in a 3 ml reaction volume |
670778 |
| 3.4.24.75 | -999 |
- |
169 U/mg (prolysostaphin), 760 U/mg (mature lysostaphin), 1 unit is defined as the amount of enzyme that causes 50% reduction of turbidity in 10 min at 37 C of an inactivated Staphylococcus carnosus TM300 cell suspension |
31499 |
| 3.4.24.75 | -999 |
- |
970 U/mg |
669553 |
| 3.4.24.75 | -999 |
- |
activities of three enzymes namely, glycylglycine endopeptidase, endo-beta-N-acetyl glucosamidase and N-acteyl muramyl-L-alanine amidase. Lysostaphin rapidly lyses actively growing and non-dividing cells including staphylococci in biofilms. Glycylglycine endopeptidase lyses staphylococcal cells by hydrolyzing glycylglycine bonds in the polyglycine bridges which form cross links between glycopeptide chains in the cell wall peptidoglycan of Staphylococcus aureus cells. Lytic principle of lysostaphin is a peptidase which liberates N-terminal glycine and alanine from Staphylococcus aureus cell wall. Hexosaminidase is present in lysostaphin preparation is specific for the gluocainyl-muramic acid bond of the bacterial carbohydrate backbone |
695804 |
| 3.4.24.75 | -999 |
- |
assay method with N-acetylhexaglycine as substrate |
31504 |
| 3.4.24.75 | -999 |
- |
depending on the purification step the specific activity varied between 300 U/mg and 1500 U/mg |
667748 |
| 3.4.24.75 | -999 |
- |
FVB albino dams infected with methicillin-resistant Staphylococcus aureus for analysis of lysostaphin impact on pharmacokinetics, minimum inhibitory concentration(0.008 microg/ml) and minimum bactericidal concentration (0.015 microg/ml) |
700580 |
| 3.4.24.75 | -999 |
- |
gene product 13 is a protein at the tail tip of the Bacillus subtilis bacteriophage phi29 with D,D-endopeptidase functionality |
699580 |
| 3.4.24.75 | -999 |
- |
in vitro activity of recombinant lysostaphin against Staphylococcus aureus isolates from hospitals in Beijing, China, with MIC values of 30-2000 ng/ml, overview |
683766 |
