EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   3.4.22.45 | -999 |
- |
amino acid substitutions in the helper component protease HC-Pro evaluated for effects on virus transmission by the wheat curl mite Aceria tosichella, vector transmission shown to be abolished after alanine substitution at cysteine residues 16, 46 and 49, measurement by determination of infectivity rate |
683105 |
| 3.4.22.45 | -999 |
- |
characterization of functional domains of helper component protease HC-Pro involved in RNA-silencing suppression activity determined, systematic analysis of silencing suppression function of helper component protease HC-Pro using pentapeptide-insertion scanning mutagenesis presented, regions with different classes of silencing-suppression activity defined, functional map with information on structural domains, biological functions and mutations affecting silencing-suppressor function or viral synergism indicated |
683718 |
| 3.4.22.45 | -999 |
- |
HC-Pro down-regulates the accumulation of 3' secondary siRNA, but not 5' secondary and primary siRNA, HC-PRO regulates the accumulation of different siRNAs |
701388 |
| 3.4.22.45 | -999 |
- |
infectivity assays described, GUS assay performed to assess ability of mutants of HC-Pro protein establishing primary infection foci on inoculated leaves, mutants generated of helper component protease HC-Pro protein summarized, proteinase assays performed to determine whether substitution mutations of HC-Pro protein that altered pathogenicity phenotype also affect auto-proteolysis, in all attenuated mutants retained auto-proteolytic activity of HC-Pro protein observed, activity reduced or absent among mutants unable to establish infection foci |
683717 |
| 3.4.22.45 | -999 |
- |
interaction studies of the HC-Pro protein of potato virus Y with the 20S proteasome complex from Arabidosis thaliana, interaction in living cells confirmed, N-terminal region (residues 1 to 97), central region (residues 98 to 298) and C-terminal region (residues 299 to 456) assigned, N-terminal region of HC-Pro found to be involved in binding to the 20S proteasome complex, virus transmission process and in the interaction with host proteins |
683832 |
| 3.4.22.45 | -999 |
- |
involvement of helper component protease HC-Pro in necrotic symptom expression in broad bean Vicia faba indicated, characterization of a spontaneous mutant (MM) that does not induce lethal necrosis on broad bean assigned to helper component protease HC-Pro, mutations T27I and D193Y assigned to putative zinc finger domain and to the N-terminal RNA binding domain, respectively, RNA-silencing suppression activity of helper component protease HC-Pro in Nicotiana benthamiana shown to be weakened by both mutations, T27I and D193Y, respectively |
683106 |
| 3.4.22.45 | -999 |
- |
mutant analysis of the conserved FRNK domain in the helper component proteinase HC-Pro, mutation in the FRNK motif of HC-Pro protein attenuates symptoms after virus infection without reducing initial HC-Pro protein levels, FRNK domain identified as a probable point of contact with siRNA and miRNA duplexes, analyzed by small RNA-specific microarray, mobility shift assays and GFP suppression assay, sequence similarity of FRNK motif in potyviruses compared |
683833 |
| 3.4.22.45 | -999 |
- |
TEV virion binding activity of the recombinant enzyme expressed in Pichia pastoris, overview |
669681 |
| 3.4.22.45 | -999 |
- |
zucchini yellow mosaic virus carried within the stylets of aphids, in vitro association between the potyviral helper component protease HC–Pro protein and a component of the aphid cuticle of the aphid Myzus persicae identified, protein extracts with two HC–Pro proteins, differing by the presence of amino acid residues K or E in the KLSC domain used for binding studies, HC–Pro protein with KLSC but not with ELSC found to bind proteins of Myzus persicae, role of the HC-Pro protein as a putative aphid cuticular receptor suggested |
683719 |
