EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
3.1.3.76 | -999 |
- |
optimization of activity assay conditions with the recombinant enzyme, maximal in 25 mM Tris buffer, pH 7.0, 30°C, 1 mM MgCl2 |
657156 |
3.1.3.76 | 0.007 |
- |
purified recombinant mutant R103C/R287Q, substrate 4-nitrophenyl phosphate |
660799 |
3.1.3.76 | 0.015 |
- |
purified recombinant mutant K55R, substrate 4-nitrophenyl phosphate |
660799 |
3.1.3.76 | 0.016 |
- |
purified recombinant mutant C154Y, substrate 4-nitrophenyl phosphate |
660799 |
3.1.3.76 | 0.017 |
- |
purified recombinant mutant R103C, substrate 4-nitrophenyl phosphate |
660799 |
3.1.3.76 | 0.021 |
- |
purified recombinant mutant R287Q, substrate 4-nitrophenyl phosphate |
660799 |
3.1.3.76 | 0.029 |
- |
purified recombinant wild-type allele MFA enzyme and mutant E470G, substrate 4-nitrophenyl phosphate |
660799 |
3.1.3.76 | 0.03 |
- |
purified recombinant enzyme, substrate isopentenyl phosphate |
660928 |
3.1.3.76 | 0.03 |
- |
purified recombinant mutant V422A, substrate 4-nitrophenyl phosphate |
660799 |
3.1.3.76 | 0.0954 |
- |
purified recombinant enzyme |
657156 |