EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   3.1.1.74 | -999 |
- |
- |
208319, 208325, 208330 |
| 3.1.1.74 | -999 |
- |
Assay of enzymatic activity of the fusion mutant of cutinase (RpoS-CUT) shows the same selective bioactivity as native cutinase to degrade p-nitrophenyl butyrate (PNB) but not to degrade p-nitrophenyl butyrate. |
691484 |
| 3.1.1.74 | 0.004 |
- |
4-nitrophenyl palmitate, esterase activity of the recombinant THCUT1 protein against different chromogenic substrates |
691941 |
| 3.1.1.74 | 0.12 |
- |
in 1% olive oil, esterase activity values decreased after 4 h of incubation and are 1.8, 1.1, and 0.12 micromol/min/mg against 4-nitrophenyl palmitate, at 4, 8 and 24 h |
691941 |
| 3.1.1.74 | 0.218 |
- |
pH 8.0, 50°C, purified recombinant His-tagged enzyme, 4-nitrophenyl (16-methyl sulfoyl ester) hexadecanoate |
729000 |
| 3.1.1.74 | 0.25 |
- |
4 h cultures against 4-nitrophenyl palmitate |
691941 |
| 3.1.1.74 | 0.395 |
- |
pH 8.0, 50°C, purified recombinant His-tagged enzyme, 4-nitrophenyl (16-methyl sulfone ester) hexadecanoate |
729000 |
| 3.1.1.74 | 0.4 |
- |
4-nitrophenyl valerate, esterase activity of the recombinant THCUT1 protein against different chromogenic substrates |
691941 |
| 3.1.1.74 | 0.74 |
- |
4-nitrophenyl butyrate, esterase activity of the recombinant THCUT1 protein against different chromogenic substrates |
691941 |
| 3.1.1.74 | 0.85 |
- |
in 1% olive oil, esterase activity values decreased after 4 h of incubation and are 11.4, 5.4, and 0.85 micromol/min/mg against 4-nitrophenyl butyrate, at 4, 8 and 24 h |
691941 |
