EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
2.5.1.17 | -999 |
- |
activities of recombinant truncated enzyme versions |
659058 |
2.5.1.17 | -999 |
- |
activity of mutant enzymes, overview |
674510 |
2.5.1.17 | -999 |
- |
data for ATR inclusion bodies from the enzyme expressed in E. coli with no mitochondrial targeting sequence also available |
636875 |
2.5.1.17 | -999 |
- |
nucleotide substrate specificty |
674576 |
2.5.1.17 | -999 |
- |
substantially reduced Vmax for mutants G63E, D64G, S68F, K78R, G97R and D218N |
690933 |
2.5.1.17 | -999 |
- |
wild-type kinetics by mutants G97E, C119Y, T161I, and H183Y |
690933 |
2.5.1.17 | 0.000125 |
- |
activity measured using washed ribosomes |
636866 |
2.5.1.17 | 0.000125 |
- |
pH 8.0, 37°C |
636866 |
2.5.1.17 | 0.0005 |
- |
in presence of 1 mM ATP, 20 mM NADH, 2 mM FMN, 0.5 mM hydroxycobalamin and NAD(P)H: flavin oxidoreductase, 1.5 mM MgCl2, 100 mM KCl, pH 6 |
690964 |
2.5.1.17 | 0.001 |
- |
purified enzyme, pH 8.0 |
674277 |