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EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.87-999 - mutant AAA, 20 or 200 mM lactose as acceptor, 14 and 41% galactosyltransferase activity (UDP-galactose as donor), 4 and 6% N-acetylgalactosamine transferase activity (UDP-N-acetylgalactosamine as donor), 37°C, MnCl2, 25 mM Tris-HCl, pH 7.0 707599
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.87-999 - mutant AGG, 20 or 200 mM lactose as acceptor, 0.4 and 1% galactosyltransferase activity (UDP-galactose as donor), 0.3 and 2% N-acetylgalactosamine transferase activity (UDP-N-acetylgalactosamine as donor), 37°C, MnCl2, 25 mM Tris-HCl, pH 7.0 707599
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.87-999 - mutant GAG, 20 or 200 mM lactose as acceptor, 5 and 27% galactosyltransferase activity (UDP-galactose as donor), 4 and 6% N-acetylgalactosamine transferase activity (UDP-N-acetylgalactosamine as donor), 37°C, MnCl2, 25 mM Tris-HCl, pH 7.0 707599
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.87-999 - mutant LGG, 20 or 200 mM lactose as acceptor, 2 and 1% galactosyltransferase activity (UDP-galactose as donor), 3 and 2% N-acetylgalactosamine transferase activity (UDP-N-acetylgalactosamine as donor), 37°C, MnCl2, 25 mM Tris-HCl, pH 7.0 707599
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.87-999 - mutant SGG, 20 or 200 mM lactose as acceptor, 5 and 5% galactosyltransferase activity (UDP-galactose as donor), 2 and 11% N-acetylgalactosamine transferase activity (UDP-N-acetylgalactosamine as donor), 37°C, MnCl2, 25 mM Tris-HCl, pH 7.0 707599
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.87-999 - mutant TGG, 20 or 200 mM lactose as acceptor, 3 and 15% galactosyltransferase activity (UDP-galactose as donor), 1 and 6% N-acetylgalactosamine transferase activity (UDP-N-acetylgalactosamine as donor), 37°C, MnCl2, 25 mM Tris-HCl, pH 7.0 707599
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.87-999 - mutant VGG, 20 or 200 mM lactose as acceptor, 4 and 16% galactosyltransferase activity (UDP-galactose as donor), 0.5 and 3% N-acetylgalactosamine transferase activity (UDP-N-acetylgalactosamine as donor), 37°C, MnCl2, 25 mM Tris-HCl, pH 7.0 707599
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.87-999 - relative activity: 100% wild-type, 0.1% mutant E317A, 0.4% mutant E317D, 0.8% mutant E317C, 0.1% mutant E317H 691043
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.87-999 - scale up, recombinant enzyme 489478
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.870.001 - membrane fraction 489471
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