EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
2.4.1.170 | -999 |
- |
- |
488802 |
2.4.1.170 | -999 |
- |
as glucosyl acceptor genistein shows the highest activity (100%), daidzein has 73%, formononetin shows 67%, quercetin 32%, kaempferol 19%, 4,2',4',6'-tetrahydoxychalcone 15%, apigenin 14%, aureusidin 5%, esculetin 4% and naringenin shows 3% activity (all tested with UDP-glucose). Enzyme assay is run at pH 8.5 at 30°C |
674845 |
2.4.1.170 | -999 |
- |
GmUGT1 activity with other flavonoids, overview |
736973 |
2.4.1.170 | -999 |
- |
Vmax for eriodictyol is 333.3 pkat/mg, 333.3 pkat/mg for kaempferol and 250 pkat/mg for quercetin. Assay at pH 7.0 and 37°C |
672670 |
2.4.1.170 | 0.045 |
- |
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme |
736973 |
2.4.1.170 | 0.049 |
- |
substrate daidzein, pH 8.5, 30°C, purified recombinant enzyme |
736973 |
2.4.1.170 | 0.158 |
- |
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme |
736973 |
2.4.1.170 | 0.209 |
- |
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme |
736973 |
2.4.1.170 | 0.253 |
- |
substrate daidzein, pH 8.5, 30°C, purified recombinant enzyme |
736973 |
2.4.1.170 | 0.253 |
- |
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme |
736973 |