EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    2.1.1.140 | -999 |
- |
(S)-corytuberine conversion to magnoflorine shown by crude enzyme preparation of recombinant CNMT protein, magnoflorine biosynthesis by CYP80G2 protein and CjCNMT via (S)-corytuberine as an intermediate in vivo, CjCNMT protein catalyzes the crucial N-methylation step from (S)-coclaurine to (S)-N-methylcoclaurine in (S)-reticuline biosynthesis |
687805 |
| 2.1.1.140 | -999 |
- |
analysis of inducible expression systems, production of recombinant CMT protein from PprpB promoter 2fold to 4fold higher than that from PT7 promoter, cell growth not reduced after expression form PprpB promoter even when highly induced, propionate-inducible system efficient for protein overproduction that result in growth inhibition, auto-induction experiments performed and protein production monitored |
689880 |
| 2.1.1.140 | -999 |
- |
phylogenetic analysis reveales a monophyletic clade for (S)-coclaurine N-methyltransferase (CNMT) and (S)-tetrahydroprotoberberine cis-N-methyltransferase (TNMT), 48% identity of amino acid sequence, downstream metabolic role suggests that TNMT derives from gene duplication after more ancient recruitment of CNMT, phylogenetic relationship between CNMT and TNMT provides new insights into evolutionary recruitment of enzymes into plant alkaloid pathways |
674802 |
| 2.1.1.140 | 0.000096 |
- |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated |
15230 |
| 2.1.1.140 | 0.0001 |
- |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated |
15230 |
| 2.1.1.140 | 0.0003 |
- |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated |
15230 |
| 2.1.1.140 | 0.00065 |
- |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated |
15230 |
| 2.1.1.140 | 0.00066 |
- |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated |
15230 |
| 2.1.1.140 | 0.0007 |
- |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated |
15230 |
| 2.1.1.140 | 0.00075 |
- |
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated |
15230 |
