EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   1.8.1.B1 | -999 |
- |
1536-well based kinetic absorbance assay for thioredoxin glutathione reductase. The assay uses the catalytic reduction of DTNB by NADPH in the presence of thioredoxin glutathione reductase |
684798 |
| 1.8.1.B1 | 0.0125 |
- |
substrate: glutathione disulfide, recombinant wild-type enzyme, pH 6.9, 25°C |
764653 |
| 1.8.1.B1 | 0.0599 |
- |
substrate: thioredoxin disulfide, mutant enzyme U642C,pH 7.4, 25°C |
764653 |
| 1.8.1.B1 | 0.0947 |
- |
substrate: thioredoxin disulfide, recombinant wild-type enzyme, pH 7.4, 25°C |
764653 |
| 1.8.1.B1 | 2.19 |
- |
with GSSG as substrate, at pH 7.4 and 25°C |
726293 |
| 1.8.1.B1 | 3.33 |
- |
with thioredoxin disulfide as substrate, at pH 7.4 and 25°C |
726293 |
| 1.8.1.B1 | 4.7 |
- |
GSSG + NADPH |
659902 |
| 1.8.1.B1 | 8.11 |
- |
with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, at pH 7.4 and 25°C |
726293 |
| 1.8.1.B1 | 10.3 |
- |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH |
659902 |
| 1.8.1.B1 | 11.7 |
- |
crude extract, using GSSG as substrate, at pH 7.8 and 25°C |
725773 |
