EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
1.13.11.24 | -999 |
- |
high specific QueD activity is found in crude cell extracts when the growth medium is supplemented with NiCl2 or CoCl2, but not when Mn2+, Fe2+, Cu2+, or Zn2+ is added, the activity of the purified QueD proteins depends strongly on the nature of the metal ion cofactor |
702220 |
1.13.11.24 | 0.0012 |
- |
native enzyme, using quercetin as substrate, at 25°C, pH 7.5 |
711513 |
1.13.11.24 | 0.0276 |
- |
recombinant enzyme, using fisetin as substrate, at 25°C, pH 7.5 |
711513 |
1.13.11.24 | 1.5 |
- |
25°C, pH 7.5 |
657272 |
1.13.11.24 | 1.87 |
- |
recombinant enzyme, using tamarixetin as substrate, at 25°C, pH 7.5 |
711513 |
1.13.11.24 | 2.27 |
- |
recombinant enzyme, using quercetin as substrate, at 25°C, pH 7.5 |
711513 |
1.13.11.24 | 2.49 |
- |
recombinant enzyme, using galangin as substrate, at 25°C, pH 7.5 |
711513 |
1.13.11.24 | 4 |
- |
Fe-QueD |
702220 |
1.13.11.24 | 8 |
- |
purified recombinant Fe2+-supplemented enzyme, pH 8.0, 30°C |
742192 |
1.13.11.24 | 18 |
- |
purified recombinant Mn2+-supplemented enzyme, pH 8.0, 30°C |
742192 |