EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    1.1.1.169 | -999 |
- |
- |
286107, 286110 |
| 1.1.1.169 | -999 |
- |
3.8-fold higher specific activity with NADPH than with NADH |
286110 |
| 1.1.1.169 | -999 |
- |
activity with ketopantoic acid is 100%, with ketopantoyl lactone 6%, with oxoalacetic acid 4%, no activity with 2-ketoisovaleric acid, pyruvic acid, 3-hydroxypyruvic acid, 3-phosphohydroxypyruvic acid, 2-ketobutyric acid, 2-ketoglutaric acid, and acetaldehyde |
286104 |
| 1.1.1.169 | -999 |
- |
no activity for double mutant K176A/E256A |
286109 |
| 1.1.1.169 | -999 |
- |
no activity with pantoate, ketoisovalerate, oxaloacetate, pyruvate, 3-hydroxypyruvic acid, alpha-ketoglutarate, alpha-ketobutyrate, acetaldehyde at 0.5 mM |
286107 |
| 1.1.1.169 | 0.0126 |
- |
wild-type strain |
656330 |
| 1.1.1.169 | 0.0246 |
- |
recombinant Escherichia coli mutant strain expresing the enzyme |
656330 |
| 1.1.1.169 | 0.38 |
- |
at pH 6.5 using NADPH and ketopantoic acid |
286104 |
| 1.1.1.169 | 16 |
- |
optimal reaction conditions using NADPH |
286107 |
| 1.1.1.169 | 19 |
- |
- |
286108 |
