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EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Reference
-999
-
after a 36 h incubation, the concentrations of N-acetyl-D-neuraminic acid from the reactions utilizing 0.4, 0.8, 1.2, 1.6, 2.0, and 2.2 M pyruvate as the carbon source are 7.5 (3.75%), 26.9 (3.45%), 43.9 (21.95%), 56.0 (28%), and 61.9 mM (30.95%), respectively. When using 2.2 M pyruvate, in the first 6 h, the concentration of N-acetyl-D-neuraminic acid reaches 29.5 mM (which is about half of the maximal concentration), and the productivity of N-acetyl-D-neuraminic acid is 1.6 g/l/h, the concentration of the intermediate N-acetyl-D-mannosamine also increases gradually from 0 to 64 mM during the process
-999
-
cell density of Escherichia coli BL21 (DE3) carrying the pNA1 plasmid is 37 (OD600) after fermentation for 15 h, and the highest activity for N-acetyl-D-neuraminic acid lyase is 8.472 U/ml after fermentation for 9 h, enzyme activity is assayed in reaction solution (20 mM Neu5Ac in 50 mM potassium phosphate buffer, pH 7.5)
0.015
-
N-acetyl-D-mannosamine + pyruvate, 37°C
0.0693
-
immobilized recombinant enzyme, the activity yield of immobilized enzyme is approximately 10% of the free enzyme
0.14
-
crude cell extract, at 37°C
0.42
1.5
-
1.2
2.2
N-acetylneuraminic acid, 25°C
1.5
-
-
2.45
-
crude extract, in 50 mM potassium phosphate buffer, pH 7.5, at 37°C
Results 1 - 10 of 24 > >>