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EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Reference
-999
-
development of an assay for glucosamine 6-phosphate synthase that measures the production of glucosamine 6-phosphate by either following the consumption of acetyl-CoA spectrophotometrically at 230 nm or quantifying the free thiol with 5,5'-dithio-bis(2-nitrobenzoic acid), i.e. Ellman’s reagent in a discontinuous manner. Simple assay method, which can be adapted to 96-well microtiter plate format
0.001
-
mutant enzyme L468P
0.0047
-
-
0.00783
-
-
0.013
-
-
0.0144
-
-
0.021
-
glutamine 6-phosphate-synthetic activity, mutant V711F, pH 7.5, 25°C
0.05
-
glutamine 6-phosphate-synthetic activity, mutant W97G, pH 7.5, 25°C
0.095
-
amidohydrolysing activity of the mutant enzyme W97G using D-fructose 6-phosphate as substrate
0.16
-
-
Results 1 - 10 of 30 > >>