EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
2.2.1.6 | -999 |
- |
- |
395915, 395919, 395922, 672368, 673744 |
2.2.1.6 | -999 |
- |
assay methods |
395917 |
2.2.1.6 | -999 |
- |
effects of pyruvate and sucrose on relative ALS activity in light and dark conditions, overview |
701404 |
2.2.1.6 | -999 |
- |
isozyme substrate specificities |
671290 |
2.2.1.6 | -999 |
- |
specific activity also depends on protein concentration, with lower concentration giving lower activity, activity may be generally increased by pre-incubation at 30°C prior to assay |
658566 |
2.2.1.6 | -999 |
- |
spectrophotometric assay involving an indirect detection of the product acetolactate |
395872 |
2.2.1.6 | -999 |
- |
time lag during the production of acetolactate from pyruvate and cofactors. Time lag is temperature depended being lowest at 37°C with 250 seconds |
658365 |
2.2.1.6 | 0.0023 |
- |
pH 7.5, 37°C, purified recombinant His6-tagged mutant E85A |
733747 |
2.2.1.6 | 0.0043 |
- |
substrate: pyruvate, pH 7.5, 37°C, in the presence of 3.5 M KCl |
727336 |
2.2.1.6 | 0.03 |
- |
mutant H351Q, 37°C, pH 7.5 |
657720 |