EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    1.14.13.22 | -999 |
- |
- |
438991 |
| 1.14.13.22 | -999 |
- |
activity below 0.1 micromol/min/mg protein, substrate cyclohexanone, induced (0.1 mM isopropylthiogalactoside) cell culture extracts, BHI medium or modified Riesenberg medium, 30°C |
699080 |
| 1.14.13.22 | -999 |
- |
activity is dependent on substrate concentration and buffer conditions |
438989 |
| 1.14.13.22 | -999 |
- |
whole cell activity to produce epsilon-caprolactone from cyclohexanone: initially 72 micromol/min/g dry cells, linearly falling to 14-18 micromol/min/g dry cells, sodium-potassium phosphate buffer, pH 7.0 with glucose (transferred from modified Riesenberg medium used for cultivation), 30°C |
699080 |
| 1.14.13.22 | 0.4 |
- |
substrate cyclohexanone, induced (1.0 mM isopropylthiogalactoside) cell culture extracts, modified Riesenberg medium, 30°C |
699080 |
| 1.14.13.22 | 0.5 |
- |
substrate cyclohexanone, induced (1.0 mM isopropylthiogalactoside) cell culture extracts, BHI medium, 30°C |
699080 |
| 1.14.13.22 | 0.85 |
- |
mutant W492A, cyclohexanone, 50 mM Tris-HCl buffer (pH 9.0), 0.1 mM NADPH, 25°C |
698499 |
| 1.14.13.22 | 1.4 |
- |
H163Q mutant enzyme |
658433 |
| 1.14.13.22 | 2.1 |
- |
- |
438968, 438970, 438978 |
| 1.14.13.22 | 3.57 |
- |
with cyclohexanone as substrate, pH 7.5 |
657585 |
