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EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Reference
-999
-
-
-999
-
activity below 0.1 micromol/min/mg protein, substrate cyclohexanone, induced (0.1 mM isopropylthiogalactoside) cell culture extracts, BHI medium or modified Riesenberg medium, 30░C; whole cell activity to produce epsilon-caprolactone from cyclohexanone: initially 72 micromol/min/g dry cells, linearly falling to 14-18 micromol/min/g dry cells, sodium-potassium phosphate buffer, pH 7.0 with glucose (transferred from modified Riesenberg medium used for cultivation), 30░C
-999
-
activity is dependent on substrate concentration and buffer conditions
0.4
-
substrate cyclohexanone, induced (1.0 mM isopropylthiogalactoside) cell culture extracts, modified Riesenberg medium, 30░C
0.5
-
substrate cyclohexanone, induced (1.0 mM isopropylthiogalactoside) cell culture extracts, BHI medium, 30░C
0.85
-
mutant W492A, cyclohexanone, 50 mM Tris-HCl buffer (pH 9.0), 0.1 mM NADPH, 25░C
1.4
-
H163Q mutant enzyme
3.57
-
with cyclohexanone as substrate, pH 7.5
3.68
-
with cyclohexanone as substrate, pH 7.5
Results 1 - 10 of 25 > >>