EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
1.14.13.22 | 3.68 |
- |
with cyclohexanone as substrate, pH 7.5 |
657585 |
1.14.13.22 | 3.75 |
- |
with cyclohexanone as substrate, pH 7.5 |
657585 |
1.14.13.22 | 5.4 |
- |
H96Q mutant enzyme |
658433 |
1.14.13.22 | 6 |
- |
wild type enzyme, cyclohexanone, 50 mM Tris-HCl buffer (pH 9.0), 0.1 mM NADPH, 25°C |
698499 |
1.14.13.22 | 6.8 |
- |
substrate cyclohexanone, pH 8.0, 25°C |
724071 |
1.14.13.22 | 8 |
10 |
partially purified enzyme, substrate 4-methylcyclohexanone |
438973 |
1.14.13.22 | 9.1 |
- |
purified recombinant enzyme, substrate bicyclo[3.2.0]hept-2-en-6-one |
675461 |
1.14.13.22 | 9.2 |
- |
purified native enzyme, substrate bicyclo[3.2.0]hept-2-en-6-one |
675461 |
1.14.13.22 | 9.9 |
- |
purified enzyme |
438984 |
1.14.13.22 | 14 |
- |
purified recombinant His-tagged enzyme expressed in yeast |
438992 |