   3.2.1.7 | mycelium |
response surface methodology is used for optimization of endoinulinase production at shake-flask level from Aspergillus tritici strain BGPUP6 on raw inulin extracted from root tubers of Asparagus officinalis as carbon source with 0.001% FeSO4, 0.05% MgSO4, and 0.02% KCl. The optimal combination of other media ingredients is 3% raw inulin, 0.5% peptone, 0.5% (NH4)H2PO4, at pH 5.5 and 37°C. Maximum endoinulinase production of 25.01 IU/ml and biomass yield of 0.514 g/dry weight/50 ml are obtained after 8 days of cultivation on agitation. Method optimization and validation, detailed overview |
-, 750834 |
