7.4.2.6 | solubilization and refolding of recombinant insoluble His-tagged OppA expressed in Escherichia coli strain BL21(DE3) by dilution with 100fold volumes of refolding buffer, PBS, pH 7.4, containing 1 M urea, 10% glycerol, 0.005% Tween 20, 0.5 mM PMSF and 5 mM DTT, and maintainance under vigorous agitation at 4°C for 8 h |
686956 |