3.4.21.50 | denatured and solubilized recombinant Lys-C without N-terminal pro-peptide is refolded at 0.0.25 mg/ml in glutathione, N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS), NDSB-201 and GuHCl buffer, 4°C, 48 h, refolding protocoll overview |
755295 |