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Renatured (Commentary)
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2.4.2.1
in 50 mM sodium phosphate buffer (pH 7.5), 1 mM EDTA, 1 mM dithiothreitol and 20% (w/v) of various crowding agents like PEG3300, PEG 8000, Dextran 70, sucrose and glycerol
736246
2.4.2.1
the CXC motif is necessary to obtain complete renaturation from the unfolded state. After denaturation using 6 M guadinium HCl, wild-type is able to achieve a recovery of catalytic activity of 90%. Sequence NH2-RRCGCKD-COOH acts as in vitro catalyst of oxidative protein folding
703662
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