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Show all pathways known for 1.13.11.55Display the word mapDisplay the reaction diagram Show all sequences 1.13.11.55recombinant wild-type and mutant enzymes from Escherichia coli strain B21 inclusion bodies, purified by nickel affinity chromatography, are refolded by a four-step dialysis using 20 mM Tris/HCl, 150 mM NaCl, pH 8.14, containing 2 mM reduced glutathione, 0.02 mM oxidized glutathione, 5% glycerol, 0.005% Tween 20, and 0.1 mM ferric citrate with 6 M urea in the first step, 3 M urea in the second step, and 1 M urea in the third step 725890
Show all pathways known for 1.13.11.55Display the word mapDisplay the reaction diagram Show all sequences 1.13.11.55reversible denaturation curves with midpoints at 3.4 M guanidine-HCl and 5.9 M urea 724213
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