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EC Number	Renatured (Commentary)	Reference
1.11.1.14	recombinant apoenzyme from inclusion bodies after expression in Escherichia coli strain W3110 by solubilization with 8 M urea. Subsequent in vitro refolding of the enzyme LP is performed using 2.1 M urea, 5 mM Ca2+, 0.01 mM hemin, 0.7 mM oxidized glutathione, 0.1 mM dithiothreitol, and 0.2 mg/ml protein, at pH 8.0	765049
1.11.1.14	the optimal refolding solution contains of 0.4 M urea, 5 mM CaCl2, 0.0005 mM hemin, and 0.6 mM oxidized glutathione in Tris-HCl buffer, at pH 9.5	701072

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Release 2023.1 (January 2023)