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epimerase activity of AlgE4 preincubated with 1 mM Na2EDTA can be restored by the addition of a molar excess of Ca2+
recombinant His-tagged catalytic domain, MEP13-C5, from Escherichia coli strain BL21 CodonPlus(DE3)RIPL inclusion bodies, the protein is successfully refolded using an on-column refolding procedure
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