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EC Number
Commentary
Reference
fast dilution method in 0.7 M arginine, 1 mM EDTA, 3 mM reduced glutathione, and 3 mM GSSG at pH 8.6
fast dilution method in 20 mM NH3 and 10% (v/v) glycerol at pH 10.5 for 72 h at 4?
recombinant expression of thioredoxin-fusion enteropeptidase light chain from aggregates in 3 M guanidine-HCl, pH 8.0, in Escherichia coli strain BL21(DE3) by dilution of the protein in 0.7 M arginine-HCl, pH 8.5, 15% v/v glycerol, 3 mM reduced glutathione, and 1 mM EDTA. After 72 h at 4C the refolding solution is dialyzed for 8 h against 50 mM Tris-HCl, pH 8.0, to facilitate complete autocatalytic activation by cleaving the fusion tag
Results 1 - 3 of 3