EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    6.4.1.1 | 0.0011 |
- |
ATP |
pH 7.5, 25°C, mutant K119Q, no biotin, pyruvate carboxylation |
726972 |
| 6.4.1.1 | 0.11 |
- |
ADP |
pH 7.5, 25°C, wild-type, reverse reaction |
726972 |
| 6.4.1.1 | 0.14 |
- |
ATP |
mutant H216N, MgATP cleavage, pH 7.8, 30°C |
727040 |
| 6.4.1.1 | 0.7 |
- |
pyruvate |
mutant Q870A, pH 7.5 |
706790 |
| 6.4.1.1 | 1.08 |
- |
HCO3- |
pH 7.5, 25°C, wild-type, pyruvate carboxylation |
726972 |
| 6.4.1.1 | 1.1 |
- |
pyruvate |
mutant S911A, pH 7.5 |
706790 |
| 6.4.1.1 | 1.125 |
- |
ATP |
pH 7.5, 25°C, mutant K119Q, 10 mM biotin, pyruvate carboxylation |
726972 |
| 6.4.1.1 | 1.5 |
- |
pyruvate |
wild-type, pH 7.5 |
706790 |
| 6.4.1.1 | 1.91 |
- |
ATP |
pH 7.5, 25°C, wild-type, no biotin, pyruvate carboxylation |
726972 |
| 6.4.1.1 | 2 |
- |
ATP |
mutant R472S, pH 7.8, 30°C |
727004 |
