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EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Commentary	Reference
6.3.5.1	-999	-	more	the Vmax/KM-value for NADsyn1 with glutamine is 6.3fold higher than the Vmax/Km-value for NH3	652364
6.3.5.1	0.00074	-	deamido-NMN	pH 7.5, 30°C	715566
6.3.5.1	0.0015	-	L-Glu	mutant D656A, glutamine-dependent reaction	705873
6.3.5.1	0.051	-	NH3	mutant D656A, ammonia-dependent reaction	705873
6.3.5.1	0.15	-	L-Glu	wild-type, ammonia-dependent reaction	705873
6.3.5.1	0.22	-	NH3	mutant C176A, ammonia-dependent reaction	705873
6.3.5.1	0.42	-	L-Glu	wild-type, glutamine-dependent reaction	705873
6.3.5.1	0.68	-	L-Gln	pH 8.3, 37°C	702449
6.3.5.1	0.7	-	NH3	pH 8.3, 37°C	702449
6.3.5.1	1.6	-	ATP	pH 8.3, 37°C	702449

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Release 2023.1 (January 2023)