EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
5.4.99.25 | 7.43 |
- |
tRNA uridine32 |
pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme |
702203 |
5.4.99.25 | 7.45 |
- |
tRNA uridine32 |
pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19M |
702203 |
5.4.99.25 | 12.2 |
- |
tRNA uridine32 |
pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19R |
702203 |
5.4.99.25 | 709.8 |
- |
tRNA uridine32 |
pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20G |
702203 |
5.4.99.25 | 821.9 |
- |
tRNA uridine55 |
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe. wild-type enzyme |
648867 |
5.4.99.25 | 825 |
- |
tRNA uridine55 |
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C193V |
648867 |
5.4.99.25 | 887 |
- |
tRNA uridine55 |
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A/C174A/C193A |
648867 |
5.4.99.25 | 1006 |
- |
tRNA uridine32 |
pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20L |
702203 |
5.4.99.25 | 1260 |
- |
tRNA uridine55 |
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C174A |
648867 |
5.4.99.25 | 1469 |
- |
tRNA uridine55 |
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A |
648867 |