EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    3.5.5.7 | 0.00328 |
- |
2-butenenitrile |
mutant enzyme W146F, at pH 7.0 and 30°C |
734577 |
| 3.5.5.7 | 0.0239 |
- |
2-butenenitrile |
wild type enzyme, at pH 7.0 and 30°C |
734577 |
| 3.5.5.7 | 0.141 |
- |
3-Cyanopyridine |
wild type enzyme, at pH 7.0 and 30°C |
734577 |
| 3.5.5.7 | 0.243 |
- |
3-Cyanopyridine |
mutant enzyme W146F, at pH 7.0 and 30°C |
734577 |
| 3.5.5.7 | 0.307 |
- |
3-Cyanopyridine |
mutant enzyme W146H, at pH 7.0 and 30°C |
734577 |
| 3.5.5.7 | 0.357 |
- |
3-Cyanopyridine |
mutant enzyme W146Y, at pH 7.0 and 30°C |
734577 |
| 3.5.5.7 | 0.483 |
- |
glutaronitrile |
at 30°C in 50 mM sodium phosphate buffer (pH 7.5) and 1 mM dithiothreitol |
710984 |
| 3.5.5.7 | 0.531 |
- |
Benzonitrile |
mutant enzyme W146F, at pH 7.0 and 30°C |
734577 |
| 3.5.5.7 | 0.537 |
- |
Benzonitrile |
wild type enzyme, at pH 7.0 and 30°C |
734577 |
| 3.5.5.7 | 0.689 |
- |
Fumaronitrile |
mutant enzyme W146Y, at pH 7.0 and 30°C |
734577 |
