EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
   3.4.22.69 | 0.012 |
- |
Dabcyl-KTSAVLQSGFRKME-EDANS |
mutant enzyme N28A, in 10 mM sodium phosphate, 10 mM sodium chloride, 1 mM EDTA, 1 mM TCEP, pH 7.4, at 25°C |
717244 |
| 3.4.22.69 | 0.06 |
- |
Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide |
His-tagged artificial polyprotein (cyan fluorescent protein-SARS-CoV 3CLpro-yellow fluorescent protein) |
709130 |
| 3.4.22.69 | 0.11 |
- |
Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide |
His-tagged artificial polyprotein (SARS-CoV 3CLpro-yellow fluorescent protein) |
709130 |
| 3.4.22.69 | 0.13 |
- |
Abz-LQSGFRK(Dnp)NH2 |
in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C |
717308 |
| 3.4.22.69 | 0.31 |
- |
Abz-SAVLQSGK(Dnp)NH2 |
in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C |
717308 |
| 3.4.22.69 | 0.53 |
- |
Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide |
SARS-CoV 3CLpro |
709130 |
| 3.4.22.69 | 0.643 |
- |
Dabcyl-KTSAVLQSGFRKME-EDANS |
wild type enzyme, in 10 mM sodium phosphate, 10 mM sodium chloride, 1 mM EDTA, 1 mM TCEP, pH 7.4, at 25°C |
717244 |
| 3.4.22.69 | 0.877 |
- |
Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide |
E166A mutant protein |
707753 |
| 3.4.22.69 | 1.2 |
- |
Abz-VLQSGFRK(Dnp)NH2 |
in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C |
717308 |
| 3.4.22.69 | 2.83 |
- |
Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide |
wild-type protein |
707753 |
