EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
3.4.22.60 | 0.009 |
- |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide |
mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and 195-200 |
710479 |
3.4.22.60 | 0.83 |
- |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide |
mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and inserted after Asp-203 |
710479 |
3.4.22.60 | 4.3 |
- |
N-acetyl-PEVD-7-amido-4-trifluoromethylcoumarin |
pH 7.2, 37°C |
752776 |
3.4.22.60 | 4.7 |
- |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide |
mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and 195-200, processed with thrombin |
710479 |
3.4.22.60 | 30 |
- |
Ac-DEVD-7-amido-4-methylcoumarin |
Y230V/W232Y/S234V/Q276D, pH not specified in the publication, temperature not specified in the publication |
752358 |
3.4.22.60 | 30 |
- |
Ac-VEID-7-amido-4-methylcoumarin |
Y230V/W232Y/S234V/Q276D, pH not specified in the publication, temperature not specified in the publication |
752358 |
3.4.22.60 | 33.3 |
- |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide |
wild-type protein |
710479 |
3.4.22.60 | 51.7 |
- |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide |
mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and inserted after Asp-203, processed with thrombin |
710479 |
3.4.22.60 | 60 |
- |
Ac-DEVD-7-amido-4-methylcoumarin |
wild-type, pH not specified in the publication, temperature not specified in the publication |
752358 |
3.4.22.60 | 86 |
- |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin |
mutant enzyme K41A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C |
732814 |