EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
   3.4.19.1 | 0.129 |
- |
2-aminobenzoyl-Ala-Leu-Phe-Gln-Gly-Pro-Phe(NO2)-Ala |
pH 7.0, 70°C, mutant enzyme D524A |
727876 |
| 3.4.19.1 | 0.2 |
- |
succinyl-AAA-4-nitroanilide |
in 25 mM Tris-HCl buffer pH 7.5, at 90°C |
720867 |
| 3.4.19.1 | 0.4 |
- |
N-acetyl-L-alanyl 4-nitroanilide |
pH 7.5, 80°C |
720431 |
| 3.4.19.1 | 0.414 |
- |
N-acetyl-Phe-2-naphthylamide |
pH 7.0, 70°C, mutant enzyme D524A, measured under first-order conditions |
727876 |
| 3.4.19.1 | 0.7 |
- |
Z-GGL-4-nitroanilide |
in 25 mM Tris-HCl buffer pH 7.5, at 90°C |
720867 |
| 3.4.19.1 | 1.3 |
- |
succinyl-GGF-4-nitroanilide |
in 25 mM Tris-HCl buffer pH 7.5, at 90°C |
720867 |
| 3.4.19.1 | 1.31 |
- |
N-acetyl-Phe-2-naphthylamide |
pH 7.0, 70°C, mutant enzyme D524N, measured under first-order conditions |
727876 |
| 3.4.19.1 | 1.87 |
- |
2-aminobenzoyl-Ala-Leu-Phe-Gln-Gly-Pro-Phe(NO2)-Ala |
pH 7.0, 70°C, mutant enzyme D524N |
727876 |
| 3.4.19.1 | 2 |
- |
N-acetyl-L-Phe-4-nitroanilide |
in 25 mM Tris-HCl buffer pH 7.5, at 90°C |
720867 |
| 3.4.19.1 | 2 |
- |
N-acetyl-L-phenylalanyl 4-nitroanilide |
pH 7.5, 90°C |
720867 |
