EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    3.2.2.16 | 0.00012 |
- |
S-methyl-5'-thioadenosine |
in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C |
731300 |
| 3.2.2.16 | 0.5 |
- |
5'-methylthioadenosine |
pH 7.5, 25°C, recombinant enzyme |
731300 |
| 3.2.2.16 | 1.2 |
- |
5'-methylthioadenosine |
pH 7.5, 25°C, recombinant enzyme |
731300 |
| 3.2.2.16 | 1.6 |
- |
S-adenosyl-L-homocysteine |
mutant enzyme M144A, in 100 mM HEPES (pH 7.5), at 25°C |
726889 |
| 3.2.2.16 | 7.8 |
- |
S-adenosyl-L-homocysteine |
mutant enzyme Y134F, in 100 mM HEPES (pH 7.5), at 25°C |
726889 |
| 3.2.2.16 | 10 |
- |
S-adenosyl-L-homocysteine |
mutant enzyme Y134A, in 100 mM HEPES (pH 7.5), at 25°C |
726889 |
| 3.2.2.16 | 15 |
- |
S-methyl-5'-thioadenosine |
mutant enzyme M144A, in 100 mM HEPES (pH 7.5), at 25°C |
726889 |
| 3.2.2.16 | 18 |
- |
S-methyl-5'-thioadenosine |
mutant enzyme V153I, at pH 7.7 and 25°C |
727044 |
| 3.2.2.16 | 23 |
- |
S-adenosyl-L-homocysteine |
mutant enzyme F107A, at pH 7.2 and 25°C |
727008 |
| 3.2.2.16 | 24 |
- |
S-methyl-5'-thioadenosine |
mutant enzyme Y134F, in 100 mM HEPES (pH 7.5), at 25°C |
726889 |
