EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    3.2.1.28 | 0.008 |
- |
alpha,alpha-trehalose |
mutant R222A/R287A, pH 7.0, 30°C |
715178 |
| 3.2.1.28 | 0.009 |
- |
alpha,alpha-trehalose |
mutant R222A, pH 7.0, 30°C |
715178 |
| 3.2.1.28 | 0.027 |
- |
alpha,alpha-trehalose |
mutant E520A, pH 7.0, 30°C |
715178 |
| 3.2.1.28 | 0.045 |
- |
alpha,alpha-trehalose |
mutant R287A, pH 7.0, 30°C |
715178 |
| 3.2.1.28 | 0.18 |
- |
alpha,alpha-trehalose |
mutant D322A, pH 7.0, 30°C |
715178 |
| 3.2.1.28 | 0.27 |
- |
alpha,alpha-trehalose |
mutant R169A, pH 7.0, 30°C |
715178 |
| 3.2.1.28 | 265.91 |
- |
alpha,alpha-trehalose |
wild type enzyme, 50°C, pH 6.5 |
750191 |
| 3.2.1.28 | 416.78 |
- |
alpha,alpha-trehalose |
mutant enzyme Y227H, 50°C, pH 6.5 |
750191 |
| 3.2.1.28 | 740.97 |
- |
alpha,alpha-trehalose |
mutant enzyme R442G, 50°C, pH 6.5 |
750191 |
| 3.2.1.28 | 1143.4 |
- |
alpha,alpha-trehalose |
mutant enzyme Y227H/R442G, 50°C, pH 6.0 |
750191 |
