EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
  2.1.1.85 | 0.00036 |
- |
synthetic peptide corresponding to residue 66-80 of actin |
wild type enzyme, at pH 10.5 and 37°C |
757747 |
| 2.1.1.85 | 0.039 |
- |
synthetic peptide corresponding to residue 66-80 of actin |
mutant enzyme N255V, at pH 10.5 and 37°C |
757747 |
| 2.1.1.85 | 0.05 |
- |
S-adenosyl-L-methionine |
mutant enzyme N278A, at pH 7.2 and 37°C |
756580 |
| 2.1.1.85 | 0.067 |
- |
beta-actin L-histidine73 |
mutant enzyme R75A, at pH 7.2 and 37°C |
756580 |
| 2.1.1.85 | 0.067 |
- |
S-adenosyl-L-methionine |
mutant enzyme R75A, at pH 7.2 and 37°C |
756580 |
| 2.1.1.85 | 0.203 |
- |
synthetic peptide corresponding to residue 66-80 of actin |
mutant enzyme N255A, at pH 10.5 and 37°C |
757747 |
| 2.1.1.85 | 0.25 |
- |
S-adenosyl-L-methionine |
mutant enzyme Y313F, at pH 7.2 and 37°C |
756580 |
| 2.1.1.85 | 0.28 |
- |
actin L-histidine73 |
wild type enzyme, at pH 10.5 and 37°C |
757747 |
| 2.1.1.85 | 0.35 |
- |
beta-actin L-histidine73 |
mutant enzyme N278A, at pH 7.2 and 37°C |
756580 |
| 2.1.1.85 | 0.63 |
- |
beta-actin L-histidine73 |
mutant enzyme Y313F, at pH 7.2 and 37°C |
756580 |
