EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.5.3.5 | 0.0076 |
- |
(S)-nicotine |
mutant Y311W, pH 7.4, 22°C |
764029 |
1.5.3.5 | 0.0077 |
- |
(S)-nicotine |
wild-type, pH 7.4, 22°C |
764029 |
1.5.3.5 | 0.018 |
- |
Nicotine |
37°C, pH not specified in the publication |
765711 |
1.5.3.5 | 0.0204 |
- |
(S)-nicotine |
mutant R274A/Y311W/C417W, pH 7.4, 22°C |
764029 |
1.5.3.5 | 0.024 |
- |
Nicotine |
37°C, pH not specified in the publication |
765711 |
1.5.3.5 | 0.048 |
- |
O2 |
pH 7.0, 25°C, mutant enzyme K287M, cosubstrate: (S)-6-hydroxynicotine |
741943 |
1.5.3.5 | 0.0877 |
- |
(S)-nicotine |
mutant R274A/Y311W/C417W, pH 7.4, 37°C |
764029 |
1.5.3.5 | 0.195 |
- |
(S)-nicotine |
pH 7.0, 40°C |
741686 |
1.5.3.5 | 2 |
- |
(S)-6-hydroxynornicotine |
pH 7.0, 25°C, mutant enzyme N166A |
741943 |
1.5.3.5 | 8.6 |
- |
(S)-6-hydroxynornicotine |
pH 7.0, 25°C, mutant enzyme Y311F |
741943 |