EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.14.13.92 | 0.00075 |
- |
Cyclopentanone |
recombinant wild-type enzyme, pH 7.4, 25°C |
765535 |
1.14.13.92 | 0.0016 |
- |
2-phenylcyclohexanone |
recombinant wild-type enzyme, pH 7.4, 25°C |
765535 |
1.14.13.92 | 0.0016 |
- |
Cyclopentanone |
recombinant mutant P253F/G254A/R258M/L443F, pH 7.4, 25°C |
765535 |
1.14.13.92 | 0.008 |
- |
2-phenylcyclohexanone |
wild type enzyme, at pH 7.5 and 37°C |
726793 |
1.14.13.92 | 0.029 |
- |
phenylacetone |
recombinant mutant P253F/G254A/R258M/L443F, pH 7.4, 25°C |
765535 |
1.14.13.92 | 0.043 |
- |
2-Octanone |
recombinant mutant R258A, pH 7.4, 25°C |
765535 |
1.14.13.92 | 0.069 |
- |
2-Octanone |
recombinant wild-type enzyme, pH 7.4, 25°C |
765535 |
1.14.13.92 | 0.216 |
- |
cyclohexanone |
mutant I67Y/P440F, pH 8.0, 45°C |
764409 |
1.14.13.92 | 0.223 |
- |
2-Octanone |
recombinant mutant R258M, pH 7.4, 25°C |
765535 |
1.14.13.92 | 0.37 |
- |
2-phenylcyclohexanone |
mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C |
726793 |