EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    1.1.1.85 | 0.009 |
- |
(2R,3S)-3-isopropylmalate |
mutant enzyme R136K, at pH 7.5 and 25°C |
738685 |
| 1.1.1.85 | 0.038 |
- |
3-(2'-methylthio)ethylmalate |
wild-type, pH not specified in the publication, temperature not specified in the publication |
725437 |
| 1.1.1.85 | 0.1 |
- |
3-(2'-methylthio)ethylmalate |
mutant F137L, pH not specified in the publication, temperature not specified in the publication |
725437 |
| 1.1.1.85 | 0.109 |
- |
(2R,3S)-3-isopropylmalate |
mutant enzyme D292N, at pH 7.5 and 25°C |
738685 |
| 1.1.1.85 | 0.12 |
- |
NAD+ |
wild-type (2R,3S)-3-isopropylmalate dehydrogenase, pH 8.0, 37°C |
711150 |
| 1.1.1.85 | 0.16 |
- |
(2R,3S)-3-isopropylmalate |
mutant R85V/Y86A, pH 8.0, 37°C |
711150 |
| 1.1.1.85 | 0.18 |
- |
(2R,3S)-3-isopropylmalate |
wild-type (2R,3S)-3-isopropylmalate dehydrogenase, pH 8.0, 37°C |
711150 |
| 1.1.1.85 | 0.192 |
- |
(2R,3S)-3-isopropylmalate |
mutant enzyme R146K, at pH 7.5 and 25°C |
738685 |
| 1.1.1.85 | 0.23 |
- |
NAD+ |
mutant R85V/Y86A, pH 8.0, 37°C |
711150 |
| 1.1.1.85 | 0.25 |
- |
NAD+ |
mutant H15Y/E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C |
711150 |
