EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.1.1.286 | 0.44 |
- |
isocitrate |
pH 8.0, 60°C, recombinant His6-tagged mutant T71V |
740021 |
1.1.1.286 | 0.45 |
- |
isocitrate |
pH 8.0, 60°C, recombinant His6-tagged mutant I82N |
740021 |
1.1.1.286 | 0.56 |
- |
NAD+ |
pH 8.0, 60°C, recombinant His6-tagged mutant I82N, with isocitrate |
740021 |
1.1.1.286 | 0.88 |
- |
NAD+ |
pH 8.0, 60°C, recombinant His6-tagged mutant T71V, with isocitrate |
740021 |
1.1.1.286 | 0.92 |
- |
isocitrate |
pH 8.0, 60°C, recombinant His6-tagged mutant L81P |
740021 |
1.1.1.286 | 0.93 |
- |
NAD+ |
pH 8.0, 60°C, recombinant His6-tagged mutant S80A, with isocitrate |
740021 |
1.1.1.286 | 1.2 |
- |
NAD+ |
pH 8.0, 60°C, recombinant His6-tagged mutant L81P, with 3-isopropylmalate |
740021 |
1.1.1.286 | 1.8 |
- |
NAD+ |
pH 8.0, 60°C, recombinant His6-tagged mutant L81P, with isocitrate |
740021 |
1.1.1.286 | 1.9 |
- |
NAD+ |
pH 8.0, 60°C, recombinant His6-tagged mutant S80A, with 3-isopropylmalate |
740021 |
1.1.1.286 | 2.2 |
- |
3-isopropylmalate |
pH 8.0, 60°C, recombinant His6-tagged mutant L81P |
740021 |