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Results 1 - 10 of 87 > >>
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Reference
33.3
-
4-hydroxy-2-oxohexanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
46.1
-
4-hydroxy-2-oxopentanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
7.8
-
4-hydroxy-2-oxopentanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
10.6
-
4-hydroxy-2-oxohexanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
0.45
-
(4S)-4-hydroxy-2-oxopentanoate
H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.34
-
(4S)-4-hydroxy-2-oxopentanoate
H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
-999
-
more
highest catalytic efficiency of BphI occurs when NADH is present, which is 25fold higher than the value obtained without nucleotides
0.00047
-
pentaldehyde
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
0.0005
-
Isobutyraldehyde
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
0.0022
-
Butyraldehyde
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
Results 1 - 10 of 87 > >>