EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
4.1.2.52 | 3.55 |
- |
(S)-4-hydroxy-2-oxopentanoate |
mutant enzyme R70K, with 0.5 mM Co2+, at pH 8.0 and 25°C |
725493 |
4.1.2.52 | 941 |
- |
(S)-4-hydroxy-2-oxopentanoate |
wild type enzyme, with 0.5 mM Co2+, at pH 8.0 and 25°C |
725493 |
4.1.2.52 | 0.03868 |
- |
3-deoxy-D-manno-oct-2-ulosonic acid |
i.e. KDO, pH 8.0, 25°C |
721601 |
4.1.2.52 | 1000 |
- |
4-hydroxy-2-oxoheptane-1,7-dioate |
using H2O as solvent, in the presence of 0.5 mM Co2+, at pH 8.0 and 25°C |
725493 |
4.1.2.52 | 1460 |
- |
4-hydroxy-2-oxohexanoate |
pH 8.0, 25°C |
721601 |
4.1.2.52 | 940 |
- |
4-hydroxy-2-oxopentanoate |
pH 8.0, 25°C |
721601 |
4.1.2.52 | 0.011 |
- |
4-hydroxy-2-oxopentanoic acid |
H45Q mutant enzyme, pH 8.0, 25°C |
722258 |
4.1.2.52 | 0.12 |
- |
4-hydroxy-2-oxopentanoic acid |
H45A mutant enzyme, pH 8.0, 25°C |
722258 |
4.1.2.52 | 930 |
- |
4-hydroxy-2-oxopentanoic acid |
wild-type, pH 8.0, 25°C |
722258 |
4.1.2.52 | 0.4 |
- |
acetaldehyde |
Kcat/Km(app) with 2-oxobutanoate as carbonyl donor, pH 8.0 and 25°C |
702377 |