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Results 1 - 10 of 20 > >>
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Reference
0.012
-
Dabcyl-KTSAVLQSGFRKME-EDANS
mutant enzyme N28A, in 10 mM sodium phosphate, 10 mM sodium chloride, 1 mM EDTA, 1 mM TCEP, pH 7.4, at 25°C
0.06
-
Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide
His-tagged artificial polyprotein (cyan fluorescent protein-SARS-CoV 3CLpro-yellow fluorescent protein)
0.11
-
Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide
His-tagged artificial polyprotein (SARS-CoV 3CLpro-yellow fluorescent protein)
0.13
-
Abz-LQSGFRK(Dnp)NH2
in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C
0.31
-
Abz-SAVLQSGK(Dnp)NH2
in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C
0.53
-
Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide
SARS-CoV 3CLpro
0.643
-
Dabcyl-KTSAVLQSGFRKME-EDANS
wild type enzyme, in 10 mM sodium phosphate, 10 mM sodium chloride, 1 mM EDTA, 1 mM TCEP, pH 7.4, at 25°C
0.877
-
Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide
E166A mutant protein
1.2
-
Abz-VLQSGFRK(Dnp)NH2
in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C
2.83
-
Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide
wild-type protein
Results 1 - 10 of 20 > >>