EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
3.4.21.9 | 0.22 |
- |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin |
mutant enzyme E136Y, at pH 8.3 and 30°C |
732855 |
3.4.21.9 | 0.23 |
- |
t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin |
mutant enzyme E136Y, at pH 8.3 and 30°C |
732855 |
3.4.21.9 | 0.23 |
- |
t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin |
wild type enzyme, at pH 8.3 and 30°C |
732855 |
3.4.21.9 | 0.41 |
- |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin |
wild type enzyme, at pH 8.3 and 30°C |
732855 |
3.4.21.9 | 0.62 |
- |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin |
mutant enzyme R213L, at pH 8.3 and 30°C |
732855 |
3.4.21.9 | 0.67 |
- |
t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin |
mutant enzyme R213L, at pH 8.3 and 30°C |
732855 |
3.4.21.9 | 0.85 |
- |
t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin |
mutant enzyme H24Q, at pH 8.3 and 30°C |
732855 |
3.4.21.9 | 0.89 |
- |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin |
mutant enzyme H24Q, at pH 8.3 and 30°C |
732855 |
3.4.21.9 | 1.81 |
- |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin |
mutant enzyme E136Y/R213L, at pH 8.3 and 30°C |
732855 |
3.4.21.9 | 2.29 |
- |
t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin |
mutant enzyme E136Y/R213L, at pH 8.3 and 30°C |
732855 |