EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.1.1.87 | 5.16 |
- |
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate |
recombinant His-tagged enzyme, pH 7.8, 20°C |
722303 |
1.1.1.87 | 8.75 |
- |
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate |
native enzyme, pH 7.8, 20°C |
722303 |
1.1.1.87 | 2.2 |
- |
3-isopropylmalate |
mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 13 |
- |
3-isopropylmalate |
mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 15 |
- |
3-isopropylmalate |
wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 46 |
- |
3-isopropylmalate |
mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.022 |
- |
D-malate |
wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 5.1 |
- |
homoisocitrate |
mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 98 |
- |
homoisocitrate |
mutant enzyme I82N, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 190 |
- |
homoisocitrate |
mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |