EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.1.1.87 | 70 |
- |
NAD+ |
mutant enzyme I82N, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.56 |
- |
NAD+ |
mutant enzyme I82N, with isocitrate as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.45 |
- |
isocitrate |
mutant enzyme I82N, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 98 |
- |
homoisocitrate |
mutant enzyme I82N, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 1.2 |
- |
NAD+ |
mutant enzyme L81P, with 3-isopropylmalate as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 41 |
- |
NAD+ |
mutant enzyme L81P, with homoisocitrate as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 1.8 |
- |
NAD+ |
mutant enzyme L81P, with isocitrate as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.92 |
- |
isocitrate |
mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 2.2 |
- |
3-isopropylmalate |
mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 310 |
- |
homoisocitrate |
mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |