EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    4.1.3.3 | 5.1 |
- |
pyruvate |
wild type enzyme, at pH 7.0 and 37°C |
749150 |
| 4.1.3.3 | 5.17 |
- |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid |
E192F mutant protein, pH 7.5, 25°C |
715921 |
| 4.1.3.3 | 5.33 |
- |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid |
E192M mutant protein, pH 7.5, 25°C |
715921 |
| 4.1.3.3 | 5.6 |
- |
N-acetylneuraminic acid |
pH 7.0, 37°C |
713843 |
| 4.1.3.3 | 5.67 |
- |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid |
E192N mutant protein, pH 7.7.5, 25°C |
715921 |
| 4.1.3.3 | 23.75 |
- |
N-acetylneuraminate |
at pH 8.5 and 37°C |
749312 |
