EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
   3.4.21.9 | 29.6 |
- |
GDDDDK-2-naphthylamide |
mutant enzyme E136Y, at pH 8.3 and 30°C |
732855 |
| 3.4.21.9 | 42 |
- |
GD4K 2-naphthylamide |
pH 8.4, 35°C |
717431 |
| 3.4.21.9 | 43.63 |
- |
GDDDDK-2-naphthylamide |
wild type enzyme, at pH 8.3 and 30°C |
732855 |
| 3.4.21.9 | 55 |
- |
formyl-Ala-Phe-Lys-4-nitroanilide |
pH 8.4, 25°C |
718359 |
| 3.4.21.9 | 61.1 |
- |
GDDDDK-2-naphthylamide |
mutant enzyme R213L, at pH 8.3 and 30°C |
732855 |
| 3.4.21.9 | 63.1 |
- |
GDDDDK-2-naphthylamide |
mutant enzyme H24Q, at pH 8.3 and 30°C |
732855 |
| 3.4.21.9 | 99.5 |
- |
GDDDDK-2-naphthylamide |
mutant enzyme E136Y/R213L, at pH 8.3 and 30°C |
732855 |
| 3.4.21.9 | 109 |
- |
formyl-Ala-Phe-Lys-4-nitroanilide |
pH 8.4, 25°C |
718359 |
| 3.4.21.9 | 137 |
- |
formyl-Ala-Phe-Arg-4-nitroanilide |
pH 8.4, 25°C |
718359 |
| 3.4.21.9 | 309 |
- |
formyl-Ala-Phe-Arg-4-nitroanilide |
pH 8.4, 25°C |
718359 |
